Click here to see a list of who has received our Gateway plasmids. If you have a request, check here first to see if you can get them from someone near you.

 

    Lawson Lab # plasmid name category antibiotic resistance strain Addgene Sequence Comments Lab of Origin
    201 pCSDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
CS2-based; for native expression; DISTRIBUTED by ADDGENE Lawson Lab
    221 pCS3MTdest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
CS3-based; for generating N-terminal myc tagged expression clone; DISTRIBUTED by ADDGENE Lawson Lab
    223 pCSegfpdest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
CS3-based; for generating N-terminal EGFP tagged expression clone; DISTRIBUTED by ADDGENE Lawson Lab
    251 pTolDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
promoterless Tol2 backbone for native expression Lawson Lab
    252 pTolMTDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
promoterless Tol2 backbone for N-terminal myc fusion expression Lawson Lab
    274 pTolEGFPDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
promoterless Tol2 backbone for N-terminal EGFP fusion expression Lawson Lab
    343 pTolfli1epDest GW destination amp/cam ccdB survival/DB3.1   yes fli1a enhancer/promoter in Tol2 backbone for native expression Lawson Lab
    371 pTolfli1epMTDest GW Dest (R1-R2) amp/cam ccdB survival/DB3.1   yes fli1a promoter in Tol2 backbone for N-terminal myc fusion expression Lawson Lab
    372 pTolfli1epEGFPDest GW Dest (R1-R2) amp/cam ccdB survival/DB3.1   yes fli1a promoter in Tol2 backbone for N-terminal EGFP fusion expression Lawson Lab
    373 pTolfli1epCherryDest GW Dest (R1-R2) amp/cam ccdB survival/DB3.1   yes fli1a promoter in Tol2 backbone for N-terminal mCherry fusion expression Lawson Lab
    362 pCSCherryDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
CS3-based; for generating N-terminal mCherry tagged expression clone; DISTRIBUTED by ADDGENE Lawson Lab
    366 pTolCherryDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
promoterless Tol2 backbone for N-terminal mCherry fusion expression Lawson Lab
    367 pTollmo2Dest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
lmo2 promoter in Tol2 backbone for native expression Lawson Lab
    368 pTollmo2MTDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
lmo2 promoter in Tol2 backbone for N-terminal myc fusion expression Lawson Lab
    369 pTollmo2EGFPDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
lmo2 promoter in Tol2 backbone for N-terminal EGFP fusion expression Lawson Lab
    370 pTollmo2CherryDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
lmo2 promoter in Tol2 backbone for N-terminal mCherry fusion expression Lawson Lab
    426 pDest Tol2pA GW Dest (R4-R3) amp/cam ccdB tolerant   yes
NO LONGER DISTRIBUTED; see plasmid 1055 Look Lab, Dana Farber
    444 pCSDest2 GW Dest (R1-R3) amp/cam ccdB tolerant Yes yes
pCS-backbone; for 2-way multisite LR to generate C-terminal fusions; DISTRIBUTED by ADDGENE Lawson Lab
    465 pTolDestR4-R2pA GW Dest (R4-R2) amp/cam ccdB tolerant   yes promoterless Tol2 backbone for 2-way multisite LR reactions; can be used for promoter/enhancer analysis Lawson Lab
    1055 pminTol R4-R3 GW Dest (R4-R2) amp/cam ccdB tolerant Yes   DISTRIBUTED by ADDGENE Chien Lab
Zebrafish Gateway Destination plasmids