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| Lawson Lab # | plasmid name | category | antibiotic resistance | strain | Addgene | Sequence | Comments | Lab of Origin | ||
|---|---|---|---|---|---|---|---|---|---|---|
| 208 | pDONR221 | DONR (P1-P2) | kan/cam | ccdB tolerant | No | used to generate open reading frame ENTRY clones | Invitrogen | |||
| 211 | pDONRP2r-P3 | DONR (P2R-P3 | kan/cam | ccdB tolerant | No | used to generate 3' ENTRY clones | Invitrogen | |||
| 213 | pDONRP4-P1r | DONR (P4-P1r) | kan/cam | ccdB tolerant | No | used to generate 5' ENTRY clones; must select for non-self-recombining preps (see here); use to generate cis elements for promoter/enhancer analysis | Invitrogen | |||
| 249 | pENTREGFP2 | GW entry (L1-L2) | kan | any | Yes | yes |
EGFP entry clone; has stop codon; will be in frame with N-term tags | Lawson Lab | ||
| 250 | pENTRDsRedEx2 | GW entry | kan | any | Yes | yes |
dsRedEx entry clone has stop codon; will be in frame with N-term tags | Lawson Lab | ||
| 268 | pENTRN3icd | GW entry (L1-L2) | kan | any |
No | Notch3 intracellular domain entry clone | Lawson Lab | |||
| 353 | pENTRbasEgfp | GW entry (L1-L2) | kan | any | Yes | yes | EGFP behind basal carp beta-actin promoter; use for enhancer analysis | Lawson Lab | ||
| 357 | p5E-bactinP | GW 5'entry (L4-R1) | kan | any | No | yes |
zebrafish beta-actin promoter 5' entry clone | Chien Lab, Univ. Utah | ||
| 440 | p3Eegfp | GW 3' entry (R2-L3) | kan | any | Yes | yes |
3' entry clone; used for C-terminal tag | Lawson Lab | ||
| 441 | p3Emcherry | GW 3' entry (R2-L3) | kan | any | No | yes |
3' entry clone; used for C-terminal tag | Lawson Lab | ||
| 443 | pENTRegfp3 | GW entry (L1-L2) | kan | any | Yes | yes |
EGFP entry clone; does not have stop codon; will be in frame with C-term entry tags. | Lawson Lab |