NEW!! Click here to see a recent list of who has received our Gateway plasmids. If you have a request, check here first to see if you can get them from someone near you.

 

 

 

    Lawson Lab # plasmid name category antibiotic resistance strain Addgene Sequence Comments Lab of Origin
    208 pDONR221 DONR (P1-P2) kan/cam ccdB tolerant No   used to generate open reading frame ENTRY clones Invitrogen
    211 pDONRP2r-P3 DONR (P2R-P3 kan/cam ccdB tolerant No   used to generate 3' ENTRY clones Invitrogen
    213 pDONRP4-P1r DONR (P4-P1r) kan/cam ccdB tolerant No   used to generate 5' ENTRY clones; must select for non-self-recombining preps (see here); use to generate cis elements for promoter/enhancer analysis Invitrogen
    249 pENTREGFP2 GW entry (L1-L2) kan any Yes yes
EGFP entry clone; has stop codon; will be in frame with N-term tags Lawson Lab
    250 pENTRDsRedEx2 GW entry kan any Yes yes
dsRedEx entry clone has stop codon; will be in frame with N-term tags Lawson Lab
    268 pENTRN3icd GW entry (L1-L2) kan any
No
Notch3 intracellular domain entry clone Lawson Lab
    353 pENTRbasEgfp GW entry (L1-L2) kan any Yes yes EGFP behind basal carp beta-actin promoter; use for enhancer analysis Lawson Lab
    357 p5E-bactinP GW 5'entry (L4-R1) kan any No yes
zebrafish beta-actin promoter 5' entry clone Chien Lab, Univ. Utah
    440 p3Eegfp GW 3' entry (R2-L3) kan any Yes yes
3' entry clone; used for C-terminal tag Lawson Lab
    441 p3Emcherry GW 3' entry (R2-L3) kan any No yes
3' entry clone; used for C-terminal tag Lawson Lab
    443 pENTRegfp3 GW entry (L1-L2) kan any Yes yes
EGFP entry clone; does not have stop codon; will be in frame with C-term entry tags. Lawson Lab
Zebrafish Gateway entry clones