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containi
    Lawson Lab # plasmid name category antibiotic resistance strain Addgene Sequence Comments Reference Lab of Origin
    208 pDONR221 DONR (P1-P2) kan/cam ccdB tolerant No   used to generate open reading frame ENTRY clones   Invitrogen
    213 pDONRP4-P1r DONR (P4-P1r) kan/cam ccdB tolerant No   used to generate 5' ENTRY clones; must select for non-self-recombining preps (see here); use to generate cis elements for promoter/enhancer analysis   Invitrogen
    211 pDONRP2r-P3 DONR (P2R-P3 kan/cam ccdB tolerant No   used to generate 3' ENTRY clones   Invitrogen
    249 pENTREGFP2 GW entry (L1-L2) kan any Yes yes
EGFP entry clone; has stop codon; will be in frame with N-term tags Villefranc et al. (2007) Dev Dyn Lawson Lab
    250 pENTRDsRedEx2 GW entry kan any Yes yes
dsRedEx entry clone has stop codon; will be in frame with N-term tags Villefranc et al. (2007) Dev Dyn Lawson Lab
    268 pENTRN3icd GW entry (L1-L2) kan any
No
Notch3 intracellular domain entry clone Villefranc et al. (2007) Dev Dyn Lawson Lab
    353 pENTRbasEgfp GW entry (L1-L2) kan any Yes yes EGFP behind basal carp beta-actin promoter; use for enhancer analysis Villefranc et al. (2007) Dev Dyn Lawson Lab
    357 p5E-bactinP GW 5'entry (L4-R1) kan any No yes
zebrafish beta-actin promoter 5' entry clone Kwan et al. (2007) Dev Dyn Chien Lab, Univ. Utah
    440 p3Eegfp GW 3' entry (R2-L3) kan any Yes yes
3' entry clone; used for C-terminal tag Villefranc et al. (2007) Dev Dyn  
    441 p3Emcherry GW 3' entry (R2-L3) kan any No yes
3' entry clone; used for C-terminal tag Villefranc et al. (2007) Dev Dyn  
    443 pENTRegfp3 GW entry (L1-L2) kan any Yes yes EGFP entry clone; does not have stop codon; will be in frame with C-term entry tags. Villefranc et al. (2007) Dev Dyn  
    201 pCSDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
CS2-based; for native expression Villefranc et al. (2007) Dev Dyn  
    221 pCS3MTdest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
CS3-based; for generating N-terminal myc tagged expression clone Villefranc et al. (2007) Dev Dyn  
    223 pCSegfpdest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
CS3-based; for generating N-terminal EGFP tagged expression clone Villefranc et al. (2007) Dev Dyn  
    251 pTolDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
promoterless Tol2 backbone for native expression Villefranc et al. (2007) Dev Dyn  
    252 pTolMTDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
promoterless Tol2 backbone for N-terminal myc fusion expression Villefranc et al. (2007) Dev Dyn  
    274 pTolEGFPDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
promoterless Tol2 backbone for N-terminal EGFP fusion expression Villefranc et al. (2007) Dev Dyn  
    343 pTolfli1epDest GW destination amp/cam ccdB tolerant Yes yes fli1a enhancer/promoter in Tol2 backbone for native expression Villefranc et al. (2007) Dev Dyn  
    371 pTolfli1epMTDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes fli1a promoter in Tol2 backbone for N-terminal myc fusion expression Villefranc et al. (2007) Dev Dyn  
    372 pTolfli1epEGFPDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes fli1a promoter in Tol2 backbone for N-terminal EGFP fusion expression Villefranc et al. (2007) Dev Dyn  
    373 pTolfli1epCherryDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes fli1a promoter in Tol2 backbone for N-terminal mCherry fusion expression Villefranc et al. (2007) Dev Dyn  
    362 pCSCherryDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
CS3-based; for generating N-terminal mCherry tagged expression clone Villefranc et al. (2007) Dev Dyn  
    366 pTolCherryDest GW Dest (R1-R2) amp/cam ccdB tolerant Yes yes
promoterless Tol2 backbone for N-terminal mCherry fusion expression Villefranc et al. (2007) Dev Dyn  
    367 pTollmo2Dest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
lmo2 promoter in Tol2 backbone for native expression Villefranc et al. (2007) Dev Dyn  
    368 pTollmo2MTDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
lmo2 promoter in Tol2 backbone for N-terminal myc fusion expression Villefranc et al. (2007) Dev Dyn  
    369 pTollmo2EGFPDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
lmo2 promoter in Tol2 backbone for N-terminal EGFP fusion expression Villefranc et al. (2007) Dev Dyn  
    370 pTollmo2CherryDest GW Dest (R1-R2) amp/cam ccdB tolerant   yes
lmo2 promoter in Tol2 backbone for N-terminal mCherry fusion expression Villefranc et al. (2007) Dev Dyn  
    426 pDest Tol2pA GW Dest (R4-R3) amp/cam ccdB tolerant Yes yes
promoterless Tol2 backbone for 3-way multisite LR reactions Kwan et al. (2007) Dev Dyn Look Lab, Dana Farber
    444 pCSDest2 GW Dest (R1-R3) amp/cam ccdB tolerant Yes yes
pCS-backbone; for 2-way multisite LR to generate C-terminal fusions. Villefranc et al. (2007) Dev Dyn  
    465 pTolDestR4-R2pA GW Dest (R4-R2) amp/cam ccdB tolerant   yes promoterless Tol2 backbone for 2-way multisite LR reactions; can be used for promoter/enhancer analysis Villefranc et al. (2007) Dev Dyn  
    466 pME-mCherry GW entry (L1-L2) kan any Yes   middle entry clone encoding monomeric Cherry red fluorescent protein
None Chien Lab, Univ. Utah
    474 p5E-basprom GW 5'entry (L4-R1) kan any
Yes   5' entry clone containing small multicloning site upstream of basal adenovirus E1b TATA element and the transcriptional start site from the carp bactin gene
None
    475 p5EfliEBSbasP GW 5'entry (L4-R1) kan any
Yes   5' entry clone containing fli1a enhancer element immediately upstream of E1b/carp bactin basal promoter
None
    477 p5E-Fse-Asc GW 5'entry (L4-R1) kan any
    5' entry clone with FseI and AscI sites
Kwan et al. (2007) Dev Dyn Chien Lab, Univ. Utah
    476 p5E-MCS GW 5'entry (L4-R1) kan any
Yes   5' entry clone with multicloning site
Kwan et al. (2007) Dev Dyn Chien Lab, Univ. Utah
    478 p5Efli1ep GW 5'entry (L4-R1) kan any
Yes Yes 5' entry clone containing chimeric fli1ep enhancer/promoter fragment; drives endothelial expression
Villefranc et al. (2007) Dev Dyn
    482 pENTRgal4 GW entry (L1-L2) kan any
    middle entry clone encoding the yeast transcription factor GAL4 None
    512 pME-mir GW entry (L1-L2) kan any
Yes   middle entry clone containing TATA element and non-coding exon, splice donor and splice acceptor sites. Within SD and SA is site for cloning pri-microRNA sequences
Nicoli et al. (2010) Nature
Hurlstone Lab
    543 p3E-2A-mcherrypA GW 3' entry (R2-L3) kan any
Yes Yes 3' entry clone encoding the viral 2A peptide sequence upstream and in frame with mCherry
Covassin et al. (2009) Dev Biol Chien Lab, Univ. Utah
    568 pME Kaede GW entry (L1-L2) kan any
    middle entry clone encoding the photoconvertible Kaede green fluorescent protein None Chien Lab, Univ. Utah
    570 p3E-spred1 3'UTR GW 3' entry (R2-L3) kan any     3' entry clone containing the zebrafish spred1 3' UTR Nicoli et al. (2010) Nature  
    574 pCS2-cherry-spred1 3'UTR GW expression amp any     expression clone encoding mCherry with the spred1 3'UTR Nicoli et al. (2010) Nature  
    597 pCSmcherry GW expression amp any Yes   expression clone encoding mCherry    
    776 pME-mir126 GW entry (L1-L2) kan any
Yes   middle entry clone containing TATA element and non-coding exon, splice donor and splice acceptor sites. Within SD and SA is the miR-126 pri-microRNA sequence
Nicoli et al. (2010) Nature
    782 pMEActMEKK GW entry (L1-L2) kan any
Yes   middle entry clone encoding an activated form of MEKK
Covassin et al. (2009) Dev Biol
    783 pCScherryActMEKK GW expression amp any
Yes   expression clone for generating mRNA encoding mcherry-actMEKK
Covassin et al. (2009) Dev Biol
    784 pTolfliepcherryactMEKK GW expression amp any
    expression clone for driving endothelial expression of mcherry-act MEKK
Covassin et al. (2009) Dev Biol
    785 pMEmAKT GW entry (L1-L2) kan any
    middle entry clone encoding a membrane tethered form of Akt
Covassin et al. (2009) Dev Biol
    786 pCSmAKT2Acherry GW expression amp any
Yes   expression clone for generating mRNA encoding membrane tethered Akt in frame with the viral 2A peptide and mCherry
Covassin et al. (2009) Dev Biol
    787 pTolfliepmAKT2Acherry GW expression amp any
    expression clone for driving endothelial expression of membrane tethered Akt in frame with the viral 2A peptide and mCherry
Covassin et al. (2009) Dev Biol
    808 p3E-mirCherry GW 3' entry (R2-L3) kan any
Yes   3' entry clone encoding mCherry; to be used together with middle entry clones (e.g. 776) encoding mirtrons.
Nicoli et al. (2010) Nature
    811 pME-klf2a GW entry (L1-L2) kan any     middle entry clone encoding zebrafish klf2a Nicoli et al. (2010) Nature  
    812 pTol-egfp-basP-fli1ep-cherry-spred1 3'UTR GW expression amp any     expression clone for driving endothelial expression of Egfp with a control 3'UTR and mCherry with the 3'UTR of spred1 Nicoli et al. (2010) Nature  
    813 pTol-fli1ep:miR-126-cherry GW expression amp any     expression clone for driving endothelial expression of a miR-126 mirtron and mCherry Nicoli et al. (2010) Nature  
Zebrafish Gateway clones