Below are links to information on the use of Gateway cloning for analysis of gene function and expression in zebrafish embryos. We have generated a number of vectors that we distribute to the zebrafish community. We also include a number of tips for Gateway cloning and preparation of Destination vectors.
Overview of Gateway cloning/making N-terminal fusions
Multisite Gateway for generating C-terminal fusions
List of available Gateway vectors (new window): Destination | Entry
Tips for using Gateway Destination vectors
Gateway slide show: Clickable Flash movie | .pdf
Chi-Bin Chien's Tol2/Gateway wiki site (a great resource!!)
We encourage other zebrafish researchers to share new ENTRY clones once they have made them. We will update our lists periodically to include new ENTRY clones as we make them. If you would like to add yours to our list, along with contact information on how to get these plasmids, feel free to let us know.