Available Lawson Lab plasmids

 

 

 

 

 

 

    Lawson Lab # plasmid name antibiotic resistance Addgene Sequence digest for riboprobe polymerase for riboprobe digest for mRNA polymerase for mRNA Comments reference
    1 pCSGreen kan (30 ug/ml) Yes yes NA
Mlu I SP6   Lawson et al. (2001) Dev 128:3675
    27 pCRplcg1CDS amp/kan Yes   Mlu I SP6

has mutation; use only for riboprobe Lawson et al. (2003) Genes Dev 17:1346
    28 pCSplcg1 amp Yes yes Stu I or Xho I T7 Not I SP6   Lawson et al. (2003) Genes Dev 17:1346
    30 pCSMTplcg1 amp   yes

Not I SP6   Lawson et al. (2003) Genes Dev 17:1346
    33 pCS2vegf121 amp Yes yes

Not I SP6   Lawson et al. (2002) Dev Cell 3:127
    34 pCS2vegf165 amp Yes yes

Not I SP6   Lawson et al. (2002) Dev Cell 3:127
    35 pCRvegf121 amp/kan Yes   Spe I
T7

  Lawson et al. (2002) Dev Cell 3:127
    36 pflk1 amp Yes   Eco RI T7 NA
 NA
   Lawson et al. (2001) Dev 128:3675
    37 pflt4 amp Yes   Eco RI T7 NA
 NA
   Lawson et al. (2001) Dev 128:3675
    38 pfli1 kan Yes   Eco RI T7 NA
 NA
   Lawson et al. (2001) Dev 128:3675
    39 pephrin-B2 amp     SalI T7 NA
 NA
   Lawson et al. (2001) Dev 128:3675
    40 ptie2 amp Yes   Eco 01091 T3 NA
 NA
   Lawson et al. (2001) Dev 128:3675
    41 pBS 119d10L amp Yes yes NA NA
 NA NA
   Lawson et al. (2001) Dev 128:3675
    46 pfli15EGFP amp     NA NA
 NA NA
   Lawson et al. (2002) Dev Biol 248:307
    100 pfliMTN3icd amp     NA
 NA
 NA
 NA
   Lawson et al. (2001) Dev 128:3675
    142 pCSvegfc amp   yes Bam HI T7 Not I SP6 has mutation; use only for riboprobe Covassin et al (2006) PNAS 103:6554
    156 pBS-Notch5#29 amp Yes yes (fragment)

Nco I (<500bp)

HindIII (>2.5Kb)

 T3 NA
 NA
 please note that this is NOT the same riboprobe template cited in Lawson et al. (2001) Dev 128:3675.  
    360 pCRkdrb amp/kan Yes   Not I
 SP6
 NA NA   Covassin et al (2006) PNAS 103:6554
    415 pCRdll4 amp/kan     Not I SP6 NA NA   Siekmann and Lawson (2007) Nature

 

 

 

 

 

 

For information on plasmid requests, click here.

Plasmids that we routinely use for riboprobe synthesis are listed in blue.

Note: with all of the plasmids we handle, we sometimes make mistakes. We recommend end sequencing to confirm the identity of received plasmids.