Promoter analysis using Gateway
We also use Gateway cloning to assay promoter or enhancer elements in zebrafish (Villefranc et al 2007). Our approach is based on similar techniques used in the C. elegans community (Hope et al. 2004; Deplancke et al. 2004). For this purpose, we generate ENTRY plasmids containing promoter elements flanked by attL4 and attR2 sites. You can use any attL1/attL2 flanked transgene as a reporter (in this example, we show generation of an egfp reporter). Usually, you will already have standard transgene L1/L2 entry clones available.