Protocols:

whole mount in situ hybridization (PDF)

preparation of DONR and DEST vectors (PDF) (necessary for vectors with attP4-attP1R or attL4-attL1R sites)

FACS microarray protocol (PDF)

 

NEW! Bacterial 1-hybrid plasmid and strain list

Etc. . .

Lawson Lab microarray database
(username: guest, password: password)

Published Plasmids (will open in new window*):

Non-Gateway

Gateway-compatible (includes Destination, Entry and expression constructs)

* - sequence files are available on these pages

Plasmid and fish line requests

Probes from Covassin et al. (2006), Dev Bio.

Overview of Gateway cloning

ALL PLASMIDS DESCRIBED IN MENG et al. Nature Biotechnology (2008) are available at ADDGENE.

Important info on using the Tg(fli1:egfp)^y1 line for in situs.