Lawson Laboratory



whole mount in situ hybridization (PDF)

Important info on using the Tg(fli1:egfp)y1 line for in situs.

preparation of DONR and DEST vectors (PDF) (necessary for vectors with attP4-attP1R or attL4-attL1R sites)

FACS microarray protocol (PDF)



Plasmid and fish line requests

Published Plasmids (will open in new window*):


Gateway-compatible (includes Destination, Entry and expression constructs)

Lawson Lab Addgene page 

* - sequence files are available on these pages


Overview of Gateway cloning