Lawson Laboratory
Research

 

 

wt
dbltg

Another benefit of the zebrafish is that their embryos are transparent. This allows an unprecedented level of direct observation during embryonic development.

We can take further advantage of the transparent embryos through the generation of transgenic zebrafish in which a particular cell type expresses a fluorescent protein. For example, the bottom panel to the right shows cranial blood vessels of a transgenic zebrafish in which an endothelial-specific promoter is driving expression of a nuclear localized Green Fluorescent Protein (EGFP) and a cytoplasmic Red Fluorescent Protein (dsRedEx).

Through the development and application of these transgenic lines, we have been able to directly observe blood vessel growth in vivo. These studies have allowed us to make novel insights into this process in vivo
Zebrafish as a model: transparent embryos